How to distinguish quartz cuvettes from general glass cuvettes?
12 Jul 2024
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- While scanning the empty cuvette within the instrument, it is observed that the glass cuvette exhibits absorption between 200nm-300nm wavelength, while the quartz cuvette demonstrates no such absorption.
- Without placing any samplesin the sample chamber and with the wavelength set at 250nm, the cuvette is placed in the sample path, and the one with an absorbance value of less than 0.07Abs is a quartz cuvette, and the other way round, it is a glass cuvette.
- The one marked with the letter “S”on the top of the cuvette is a quartz cuvette. It is also recommended to make comparison in the ultraviolet region, and those with absorption are glass cuvette
- In accordance with Archimedes' principle, the measurement of the density of cuvettes can tell whether they are quartz or glass cuvettes.
- The cuvette used for ultraviolet light and visible light is different, the quartz cuvette is used for ultraviolet light, and there is a requirement for the direction, while the glass cuvette can be used for visible light or ultraviolet light with larger wavelength. It should be noted that glass cuvettes absorb some light in the ultraviolet, which results in differences in absorbance when measured against the arrow and along the arrow. This can be verified by the absorbance of a known solution at a certain wavelength. Furthermore, in the light, the rough surface of a cuvette displays an arrow, indicating that the direction of the light path should be consistent with the direction of the arrow, that is, the arrow should point to the side of the tester. The cuvettes marked “Q” and “S” are quartz cuvettes.
- Quartz cuvette, UV wavelength range 200-400nm
- Glass cuvette, visible light wavelength range 330-1000nm
- When illuminated by an incandescent lamp, a cuvette with higher light transmission is a glass cuvette, and the inside of a quartz cuvette should be slightly
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